Comprehensive Cell Culture Media Solutions
For Your Cell-, Viral-, and Protein-Based Therapies.
Choose a winning culture for your therapy development. At Sartorius, we offer a wide range of media, raw materials, and services to meet your cell culture needs. Backed by a global manufacturing network, we provide what’s vital to your cells and your success - from screening to commercial production.
- Find the right media, raw materials, and supporting services
- Transition to GMP with compliant media and reagents
- Scale seamlessly with flexible formats
- Work with our dedicated technical and regulatory experts
- Get consistent quality from our global manufacturing network
A Winning Culture Combines What’s Vital to Your Cells — And Your Success.
A winning culture nourishes, and our comprehensive media solutions portfolio cultivates cells for your modality. We offer a wide variety of media and raw materials, along with the technical and regulatory support needed to catalyze your therapy development. Start off strong by finding the right materials and supporting services from our extensive offering.
A winning culture delivers the performance you need. Set your therapy development up for success with our proven and process-compatible media, raw materials, and services. We provide a variety of formats - powders and liquids in bottles, bags, and buckets - to help you overcome process development gaps quickly, scale seamlessly, and ensure robust performance.
A winning culture assures. Your commercial success depends on a reliable supply of high-quality materials, and our established global manufacturing and distribution network supports your needs. Sustain and scale up your manufacturing with consistent quality that minimizes product variability and meets relevant regulatory standards.
Explore Solutions by Application
Media Solutions for Your Cell-, Viral-, and Protein-Based Therapies
Sartorius offers an extensive portfolio of high-quality media, raw materials, and services to support specialized cell types used in protein-based, viral-based, and cell- and gene-based applications.
Comprehensive CHO Media, Raw Materials, and Services
Chinese hamster ovary (CHO) cells are ideal for therapeutic protein production in biopharmaceutical research and development. Our comprehensive offering of seven CHO cell culture media and four feeds, combined with our advanced cell line development and supporting media optimization services, enables simplified clone selection, optimized titer production, and seamless scale-up to your commercial needs.
Comprehensive HEK Media, Raw Materials, and Services
Maximize titer production for your viral-based media with a complete upstream solution. We support most common cell line, reagent, and serotype combinations with our four chemically defined GMP media, feed supplements, and reagents. Find solutions for your AAV, LVV, and vaccine development and manufacturing, along with custom media development, optimization, and characterization services.
Comprehensive Media Solutions for Your Cell-Based Therapy
Maximize cell quality attributes, ensure reproducible results, and reduce your development timelines. Whether you’re working in immune or stem cell therapies, we offer GMP-grade advanced therapy cell culture media, critical raw materials, as well as storage and preservation solutions to cover all major cell types.
Explore Media, Reagents, and Supplements
Learn More About Cell Culture Media
FAQs - Cell Culture Media
A primary cell line is produced from a single cell type isolated from tissue and cultured under optimal and controlled conditions.
Cell medium ingredients include nutrients (both organic and inorganic), vitamins, salts, O2 and CO2 gas phases, proteins (from defined or undefined sources), carbohydrates, cytokines, growth factors, and cofactors.
Media should be changed every 2 to 3 days. However, media is best changed when cell density reaches a healthy level, so this also depends on the growth rate of your cell culture. Most media also contain a pH indicator that changes color to yellow when pH drops, which is a sign of nutrient depletion and waste accumulation.
- Serum-containing media - basal media supplemented with undefined biological materials such as serum or cell extracts.
- Serum-free, animal component-containing media - basal medium supplemented with defined proteins, growth factors, and cytokines from animal sources;
- Serum-free, xeno-free medium - basal media supplemented with defined proteins, growth factors, and cytokines from human origin only.
- Animal component-free, chemically defined medium - basal medium composed of material from chemically defined sources.
Cell culture medium provides nutrients and growth factors necessary for cell proliferation. A typical basal cell culture medium is composed of amino acids, carbohydrates, vitamins, inorganic salts, glucose, trace elements, and a buffer system to maintain a stable pH. Traditionally, animal-derived serum is used to supplement basal media to provide a source of growth factors. Now, serum-free, chemically defined formulations are highly favored.
Serum-free media are media designed to grow cells in the absence of animal-derived serum. Serum-free media include chemically defined media, which are free of any animal components or hydrolysates.
Serum-free media allows cell culture to be performed under a defined set of conditions, limiting batch-to-batch variability. It also simplifies purification and downstream processing.
Xeno, derived from the Greek word “xenos”, means “foreigner.” A xeno-free media is a culture medium with a formulation that only contains human-derived components and not any other species such as bovine or porcine.
Serum-free media are free from any serum (from all animal species) but can include other animal components (such as HSA and BSA), while xeno-free is free of components from non-human animal species but can include human serum.
Each medium should be stored according to the manufacturer’s protocols and recommendations. Most basal media require storage under controlled conditions at 2-10°C, light protected, and secured to avoid degradation and contamination of the components. Once opened and supplemented with additional components under sterile conditions, the medium can typically be stored for 1-4 weeks at 2-8°C and light protected.
Each medium should be stored according to the manufactured protocols and recommendations. The typical expiration date is within 12-18 months of manufacture. When open and supplemented with other components under sterile conditions, media can generally be stored for 1-4 weeks, at 2-8°C, and protected from light. Classical media supplemented with serum antibiotics can be kept at 4°C for up to 4 weeks; serum-free media can be kept for up to 2 weeks.
Bleach should be added to unused media at ≥10% final concentration to treat any microorganism present before disposal.