Flow Cytometry Instruments by Sartorius
The iQue® 3 Advanced Flow Cytometry System — Go from Samples to Actionable Results in Record Time
Traditional flow cytometers have been good at capturing a small number of samples and performing in-depth analysis on either the phenotypes or the captured signals in separate assays due to the wide range of fluorescent signals. This makes it necessary to run separate assays at differing time points, reducing biological relevance and making it difficult to compile and merge the data for interpretation.
The iQue® advanced flow cytometry platform utilizes a fixed wide dynamic range allowing for the collection of both the phenotypes and functional analysis of secreted cytokines simultaneously, eliminating the discrepancy of different time points or the need to split samples for subsequent analysis.
Watch the iQue® 3 In Action
Watch the iQue® 3 Advanced Flow Cytometry System In Action
System Overview: iQue® 3 Advanced Flow Cytometry
- A simple, scalable, multi-user environment featuring walkaway automation, comprehensive analysis and visualization tools that are not available in traditional flow cytometers
- Walk-up-and-use operation that eliminates the need for pre-analytical user setup and power users
- Flexible plate formats suitable for a broad range of cell and bead-based analyses
Rapid, Walkaway Processing of 96 or 384 Well Plates
- Patented, rapid microvolume sampling processes 96 well plates in as few as 5 minutes or 384 well plates in 20 minutes
- Samples are delivered to detectors in an air-gap delimited stream, so an entire plate of data can be processed at one time
- Generous cartridge station allows for unattended multiple plate processing
Explore iQue® Cell Phenotyping and Secreted Protein Detection within the Same Well
Real-time Data Acquisition and Analysis and Dynamic Visualization of Results
- Rapid assay optimization – adjust a gate to see updated plate-wide analysis in real time
- Easy, interactive assay development and decision environment that is reproducible across plates
- Plate level annotation, analysis, and visualization integrated within the standard population selection tools
See What Sartorius Has to Offer In Flow Cytometry
Biologically Relevant, Reproducible, and Cost Effective Flow Cytometry
Flow Cytometry Applications
Advanced Flow Cytometry Applications to Support Your Most Important Research
To efficiently characterize disease states and to develop a clear picture of how single cells respond to a stimulus, whether it’s a small molecule, antibody, cytokine, or other entity, quantitative measurement of multiple biological readouts is required.
iQue® Flow Cytometry Applications enable the assessment of multiple cell types and a range of cellular parameters in a single well, allowing for a streamlined workflow, improved data coherence and a highly informed data set.
Request a Quote or Demo Explore All Flow Cytometry Applications
Antibody Characterization
Increase data throughput and quality by multiplexing antibody binding, function, and titer across the process.
Cytokine Profiling
Assess multiple cell parameters faster, with fewer cells and less reagents.
Cell Monitoring and Workflows
Streamlined combined workflows and protocols that are simple and easy to follow providing greater biological insights
Immune Cell Function
Understand the Complexities of the Immune System with the iQue® Advanced Flow Cytometry Platform
Cell Health & Proliferation
Analyze cell health, viability and proliferation on a cell-by-cell basis with the iQue® Advanced Flow Cytometry Platform
Flow Cytometry Software, Reagents, and Consumables
Flow Cytometry Software
iQue® Forecyt® Software —Rapid Data Visualization and Interpretation
The integrated iQue® Forecyt® software addresses the analysis bottlenecks associated with multi-parametric data generation by enabling rapid data visualization and interpretation.
Reduce data analysis times from days to minutes by having all of your data analyzed as soon as the plate is done sampling.
Flow Cytometry Reagents & Consumables
Optimized for the iQue® Advanced Flow Cytometry System
iQue® Kits, most of which have been developed to work with no-wash protocols, are packaged to suit a wide range of throughput needs.
These kits, in conjunction with an iQue® Advanced Flow Cytometry Platform and iQue Forecyt® Software, provide an integrated screening solution enabling high content, high throughput screening of cells and beads for deeper insight into complex biologies.
iQue® Specifications & Optical Configurations
The iQue® 3 is Available In Multiple Optical Configurations and Flexible Plate Formats
The iQue® 3 flow cytometry platforms come in 3 configurations to address a wide range of experimental applications. The entry level 2 laser iQue® 3 BR system is equipped with a blue and a red laser ideal for applications which do not require many parameters, but, can take advantage of the speed the iQue® platform is famous for.
Two additional 3 laser configurations increase the flexibility and accommodate larger multiplexing needs depending on the specific fluorphores. The iQue® 3 VBR system includes Violet, Blue, and Red lasers, while, the iQue® 3 VYB replaces the red laser with a yellow laser.
iQue® 3 Blue-Red (BR) | iQue® 3 Violet-Blue-Red (VBR) | iQue® 3 Violet-Yellow-Blue (VYB) | |
Catalog No. | 91375, 91381 (HD) | 91377 | 91379 |
Lasers | 488nm, 640nm | 405nm, 488nm, 640nm | 405nm, 561nm, 488nm |
Detection Channels | 8 | 15 | 15 |
Plate Types | 96- and 384-well, 384- and | 96- and 384-well | 96- and 384-well |
Min Sampling Time | < 5 minutes/96 wells, < 20 minutes/384 wells | ||
Min Sample Aspiration | 1 µL | ||
Detection Rate | Up to 35,000 events/second | ||
Dynamic Range | > 7 Decades | ||
Optional Products | Cat. No. |
|---|---|
Forecyt® Enterprise Edition with 5 Floating Client Licenses | 91131 |
Robotic Integration API License | 90208 |
Qmax Refill Module | 91309 |
See What iQue Users Are Saying
Scientist ModiQuest Research
In-solution, cell-based assays are the way forward and the ability to do so with the iQue Screener in a 384-well high-throughput format has been extremely useful to us. We can now use one third of the materials and screen 4 times more antibody clones in the same amount of time, obtaining more compelling data for ourselves and our clients.
Flow Cytometry Instrument Service
Service Contract Program for the iQue® Platform
Stay current, ensure productivity, manage costs and avoid downtime
Keeping your instrument covered under a Sartorius service contract is an important part of ensuring productivity, managing cost and avoiding downtime. A Sartorius service contract has these key benefits:
- Full coverage for parts, labor, shipping and travel in event of repair.
- The use of a loaner instrument while repairs are made
- Major version software updates
- Unlimited email and voice tech support
Additional Resources
iQue® Advanced Flow Cytometry Publications
With over 200 references worldwide, the iQue®️ Advanced Flow Cytometry Platform helps researchers explore new areas of study and address questions fundamental to life science research.
Fluorescence Spectraviewer
Use the dynamic, interactive fluorescence spectraviewer tool to see compatibility with over 1,000 fluorophores on the different iQue® 3 configurations.
Frequently Asked Questions
Sartorius Flow Cytometry Instrument FAQs
Get answers to the most common questions about about Sartorius flow cytometry instruments including critical information regarding measuring, speed, plate handling, compliance, and more.
Cell or particle size and complexity are the non-fluorescent measurements along with fluorescent detection which can be used to identify and quantitate specific proteins both on the surface of cells as well as detection of secreted proteins or signaling molecules.
The iQue® flow cytometer uses a proprietary Air-Gap sampling method which can sample an entire 96 well plate in 5 minutes, or a 384 well plate in 15 minutes. But, the speed doesn’t stop there. The included Forecyt® software makes analysis a breeze by utilizing dynamic plate level data analysis and visualization making quick work of identifying the samples of interest.
Yes. It is compatible with all the major automation vendors systems. An iQue® 3 automation client software license is available allowing the plate handling robot to control the iQue® 3 to load plates, begin a run, and remove plates when the run is completed.
The enhanced rinse station is a smart fluidics system that constantly measures the volume of each buffer or solution to avoid failed runs due to insufficient reagents. It utilizes special cartridges for each fluid which minimize evaporation and can be sealed and placed in the refrigerator when not in use to extend the on-board lifetime when not in use.
Yes. There is a software module available which provides support for labs which require an additional level of traceability for experiments run on the iQue® platform. Among other features, it includes customizable user roles to help tailor the level of documentation and access to information based on specific needs or functions.