Incucyte® S3 Live-Cell Analysis Instrument

See What Your Cells Are Doing and When They Do It With the Incucyte® S3 Live-Cell Analysis Instrument

Derive deeper and more physiologically relevant information about your cells – plus real-time kinetic data. The Incucyte® automatically acquires and analyzes images around the clock, providing an information-rich analysis that is easy to achieve.

Change can happen in an instant.  Whether simply assaying cell health or more complex processes like migration, invasion, or immune cell killing, see what happened and when—without ever removing your cells from the incubator.

Never miss powerful insights again, with the Incucyte® S3 live-cell analysis system, reagents, and consumables.

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Incucyte® S3 Features

Key Features

Incucyte® transforms research into a complete and powerful workflow that supports your entire research team — day and night.

Support for Multiple Users

  • Incucyte® accommodates up to six microplates at a time
  • Users can schedule experiments at different image acquisition frequencies and magnifications in parallel
  • Remote, networked access with unlimited, free licenses

Simple, Flexible Sample Prep

  • Compatible with a wide range of culture vessels and applications
  • Utilize Incucyte® reagents for minimal cell perturbance and maximum efficiency

Set Up & Walk Away

  • Brand new user interface for simple and flexible experimental set up
  • Track experimental conditions from setup through analysis

Acquire Images Over Days or Weeks

  • Leave your cells undisturbed in the incubator during image acquisition
  • Unique optical design – cells remain stationary while optics move
  • Multiple imaging modes – HD phase plus red and green fluorescence

View and Analyze in Real-Time...or Later

  • Efficient and reproducible image analysis
  • Powerful visualization of images and time courses

Other Key Features

Speed and Sensitivity In Mind

  • CMOS camera and efficient optics enable long-term live-cell imaging and analysis
  • 20 total core processors allowing for fast image processing and data analysis

Data Protection & Storage

  • Includes 27.3 TB of RAID hard drive data storage, which can be further extended with the 32.7 TB Incustore

LCD Control Panel

  • Monitor and perform basic status checks and operations from the front panel of the Incucyte S3 System controller unit


Live-Cell Analysis Handbook: 6th Edition

Discover how the power of artificial intelligence for label-free, live-cell imaging and analysis is moving research forward and driving deeper discove...

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Live Cell Insights Publications Newsletter

Featuring new and notable Incucyte® publications

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Live-Cell Analysis

What is live-cell analysis and what are the benefits?

What is live-cell analysis and what are the benefits?

Combine information-rich, image-based analysis with the convenience and throughput of microplate assays.

Derive deeper insights with real-time live-cell analysis. (A) Automatically acquire images over time, (B) use Incucyte® VesselView to view images of all locations in the vessel at once and quickly assess experimental results, plus zoom in on images of interest (C) automatically identify regions of interest via masks (D) generate presentation-ready timelapse graphs, and (E) view all 96- or 384-well kinetic trends at once with Incucyte® PlateGraph and export data to calculate EC50 or IC50 response values.


Ask New Questions

  • Flexible assay platform enables experiments that were not previously possible
  • Kinetic, image-based measurements provide answers to everyday and unique scientific questions

Get New Answers

  • Real-time continuous analysis means you never miss a data point
  • Profile cell-specific and time-dependent biological activity
  • Visualize and validate results with images and movies

Improve Productivity

  • Add Incucyte proprietary reagents directly to cells, and walk away as Incucyte automatically acquires and analyzes images
  • Multiplex measurements in 96- and 384-well assay formats
  • Accommodate multiple users and applications simultaneously

Protect Your Cells

  • Perform analysis without removing cells from the incubator
  • Maintain cell health and morphology with non-perturbing reagent formulations


More than 20 Incucyte® Applications!

The Incucyte® S3 System is used for real-time cell monitoring and surveillance, cell health and viability, migration and invasion, plus a wide range of phenotypic cell-based assays. With a rapidly growing collection of over 3,000 peer reviewed articles, new applications using the Incucyte® are being published continuously.

See Our Full List of Applications


See What Your Peers Are Saying About the Incucyte® System

The Incucyte® Live-Cell Analysis System is the first real-time system that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside a standard tissue culture incubator. Kinetic, image-based measurements ensure you never miss a relevant response.

"Our best Cell QC and monitoring system...which makes scientists happy!"
— Cécile Cazaubon, Novartis Pharma AG

"Excellent technology and worth the money."
— Adam Case, University Of Nebraska Medical Center

"Indispensable to our research."
— Ali Talebi, KU Leuven

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Ordering Information



Cat. No.

Incucyte® S3 Live-Cell Analysis Instrument

Includes image acquisition and analysis system with 4x, 10x, 20x objectives, controller, and unlimited software licenses for standard acquisition and basic analyzer


Incustore® S Storage Unit

Additional 32.7 TB storage for Incucyte S3 extends storage to 49 TB in total


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Related Resources

Incucyte® S3 Technical Specifications

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5th Edition Live-Cell Analysis Handbook

Real-time live-cell analysis is redefining the possibilities and workflows of cell biology.

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Incucyte - Empower Live-Cell Analysis Inside Your Incubator

Incucyte® - Empower Live-Cell Analysis Inside Your Incubator

Analyze your cells for days, weeks or even months as they sit stationary in the stable environment of your tissue culture incubator.

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smart live-cell monitoring analysis webcast

Smart Live-Cell Monitoring Analysis Webcast

If you missed our latest live session, you can now view the recording of the “Smart Cell Monitoring & Analysis” webcast along with the presentatio...

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Smart Live-Cell Monitoring Analysis White Paper

Addressing the new requirements of cellular research with real-time live-cell analysis

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Live Cell Insights Publications Newsletter

Featuring new and notable Incucyte® publications

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