Chromatography Consumables
The Right Technology for the Right Task
An evaluation of chromatography technologies requires multiple layers of decision-making. Based on the characteristics of the molecule you’re isolating and purifying, there are questions about the most suitable method, or chemistry, and the most suitable format for the stationary phase. Choosing the best solution from the start will streamline process development, scale-up, and commercialization of chromatography processes. And making this choice is easier when you work with a supplier offering a comprehensive portfolio of membranes, resins, monoliths, columns, and systems combined with deep expertise in the separation and purification of complex biomolecules.
Sartorius chromatography solutions cover the full range of separation technologies and methodologies available to accommodate any process and any molecule regardless of size and unique characteristics.
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Explore the Range of Sartorius Chromatography Solutions
Resins
For traditional column chromatography capture and polishing steps:
- Appropriate for affinity, ion exchange and mixed-mode methods
- Ideal for monoclonal antibodies and recombinant proteins
- Can save time and reduce costs by eliminating dilution and adjustment steps
Monoliths
Unique, single-unit structures for preparatory/analytical applications and immobilization screening:
- Offers wide range of ligands for capture and polishing
- Appropriate for affinity, IEX, HIC, and mixed-mode methods
- Ideal for large biomolecules (e.g., viruses, nucleic acids, and exosomes)
Membranes
Known for high throughput in capture and polishing steps for IEX, HIC, and affinity methods:
- Preferred for DNA, HCP, endotoxin removal, and virus clearance in flow-through mode
- High productivity with a scalable device format
- Capsule and cassette formats eliminate need for packing hardware
Sartorius Expertise Spans Chromatography Chemistries
Ion Exchange
IEX plays a key role in capture and purification steps:
- Separates biomolecules based on differences in net charge at a particular pH
- Allows high-speed processing without sacrificing capability
- An established, industry-proven method
Hydrophobic Interaction
HIC is regarded as a more precise purification step:
- Separates molecules based on differences in surface hydrophobicity
- Powerful aggregate removal
- Ideal for use after elution with high salt concentrations (e.g., IEX)
Affinity
Affinity offers a highly specific interaction between a target molecule and its ligand:
- Single-step reduction of major contaminants
- High binding capacities in a variety of selectivities
- Purifies a variety of biomolecules including recombinant proteins, monoclonal antibodies, and mRNA
Mixed-Mode
Multimodal separation can achieve higher selectivity and purity in a single step:
- Ideal for novel biopharmaceutical targets such as nanobodies, plural-specific mAbs, and fusion proteins
- Matrices scalable from benchtop to commercial manufacturing
Featured Chromatography Consumables
A Leader in Process Chromatography Solutions
From the introduction of Sartobind® membranes more than two decades ago to the present day, Sartorius has established itself as an industry leader in process chromatography. Our portfolio encompasses multiple purification techniques and formats, and our experts will help you make the best choice to meet your demands for flexibility, productivity, and cost-efficiency. Count on Sartorius for:
- Modular solutions for both batch and intensified processing, in both multi-use and single-use designs
- Versatile chromatography systems and fully automated, scalable columns
- A diverse family of resins, membrane adsorbers, and monoliths to address the full range of purification needs in bioprocessing
