Affinity Chromatography
Affinity chromatography offers a highly specific interaction between a target molecule and its ligand. This interaction reduces major contaminants to a low level within a single step. Sartorius offers multiple affinity chromatography matrices with high binding capacities, in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen, growth hormones, monoclonal antibodies, and mRNA.
Affinity matrices scalable from benchtop to commercial manufacturing
Affinity ligands achieve high purity for a broad range of molecules
Plug-and-play chromatography matrices allow high-speed processing without sacrificing capacity
Choose the Right Affinity Matrix for Your Application
Chromatography matrices are not one-size-fits-all. Choosing the matrix best suited for your application will ensure streamlined process development, scale-up, and manufacturing without sacrificing purification performance.
Range of ligands | Heparin, lysine, blue dye | Protein A | Oligo-dT18 |
Multi- or single-use | Multi-use | Single-use | Single-use |
Ease of use | Bulk material | Ready-to-use | Ready-to-use |
Key applications | Proteins | mAbs | mRNA |
High-Capacity Affinity Resins
Find the Right Solution for Your Affinity Chromatography Process
Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones.
Applications | Coagulation factors (ATIII), growth hormones and enzyme purification | Plasminogen, recombinant protein and glycoprotein purification | Albumin, enzymes, recombinant proteins and purification |
Dynamic binding capacity (10 % BT) | > 25 mg/mL | - | > 10 mg/ml |
Average particle size | 80 µm | 70 µm | 60 µm |
Working pH range | 3 –13 | 3 –13 | 1 – 10 |
Operating pressure | <3 barg (44 psig) | <3 barg (44 psig) | - |
Heparin HyperD®
Heparin HyperD® M resin is suited for the purification of heparin-binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.
Lysine HyperD®
Lysine HyperD® affinity resin is used for the purification of lysine-binding proteins such as plasminogen. It provides high protein-binding capacities at high flow rates.
Blue Trisacryl® M
Blue Trisacryl® M is an affinity resin used for the purification of enzymes, interferons, and some coagulation factors as well as for the removal of albumin. The resin uses Trisacryl® based beads which carry the blue dye ligand.
Affinity Membranes
Sartobind® Rapid A
Goodbye column packing, hello Sartobind® Rapid A. Meet a unique scalable, highly productive membrane platform for mAb capture.
Sartobind® Rapid A changes the game for mAb capture with a scalable, disposable and highly productive plug-and-play membrane.
Monoliths for Affinity Purification
Highly Specific Monolithic Affinity Purification
Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.
- Channel sizes: 2 µm
- Column volume: 1 mL – 8 L
- Working range, pH: 2 - 13
- Functionality: Thymine oligomer based affinity
- Application: mRNA
CIMmultus® Oligo dT – Affinity for RNA
Oligo dT ligands covalently bound to solid support
Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT
Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Selectively removes impurities from samples
Convenient approach for initial purification of mRNA
Available with:
