Chromatography Membranes
Sartobind® Membrane Adsorbers – Trusted for More Than Two Decades in Commercial Processing
Sartobind® membranes transform chromatography steps into a highly productive process, from screening to production:
- The high flow rates of these adsorptive membranes make them 150× faster than a traditional column
- The capsule and cassette formats eliminate the need for packing hardware
- The membrane can be sized to fit the impurity removal process based on capacity rather than flow rate
- Single-use consumables eliminate the need for costly cleaning validation
- Available in a variety of chemistries
Want to Remove the mAb Capture Bottleneck?
See first-hand how Sartobind® Rapid A delivers a tenfold increase in productivity with Sartorius’ new chromatography membrane. Learn more about the product’s scalability, from PD to commercial, and how to remove the mAb capture bottleneck.
Capsules can be handled like a filter, and cassettes can be installed in less than 10 minutes
From 96-well plates to 5 L capsules and modular cassettes, Sartobind® offers a linear scale-up to all process scales
High flow rates (5 – 20 MV/min) reduce processing time, a dramatic contrast to columns with 0.5 CV/min or slower flow rates
The capsule and cassette formats eliminate the need for column packing, reducing the required facility space
Single-use consumables eliminate need for cleaning validation, while the fit based on capacity vs. flow rate reduces buffer consumption
Sartobind Membranes for All Process Scenarios
Sartobind® membranes are available for both flowthrough and bind/elute applications.
Sartobind® Rapid A | Sartobind® Q | Sartobind STIC® PA | Sartobind® S | Sartobind® Phenyl | |
|---|---|---|---|---|---|
Ligand | Protein A | Quaternary ammonium | Primary amine | Sulfonic acid | Phenyl |
Functionality | Affinity Chromatography | Strong AEX | Salt-tolerant AEX | Strong CEX | Hydrophobic Interaction |
Bed height | 4 mm | 4 and 8 mm for flowthrough and bind/elute | 4 mm for flowthrough only | 4 and 8 mm for flowthrough and bind/elute | 8 mm for flowthrough and bind/elute |
Common uses | mAb capture | Endotoxin/DNA/HCP removal Virus purification | Endotoxin/DNA/HCP/virus removal | HCP/Aggregate removal | Aggregate/HCP/lipid/dye/endotoxin removal Nucleic acid purification |
Affinity Membranes
Sartobind® Rapid A
Goodbye column packing, hello Sartobind® Rapid A. Meet a unique scalable, highly productive membrane platform for mAb capture.
Sartobind® Rapid A changes the game for mAb capture with a scalable, disposable and highly productive plug-and-play membrane.
Sartobind® IEX Membranes
High Speed IEX Capture/Polishing
Sartobind® IEX membranes enable rapid purification of various biomolecules. Ready-to-use format minimizes set-up time and makes chromatography a simple and highly productive process step.
Ligand | Quaternary ammonium | Primary amine | Sulfonic acid |
IEX Type | Strong anion exchange | Salt-tolerant weak anion exchange | Strong cation exchange |
Typical DBC at 10% breakthrough | BSA: 29 mg/mL | BSA: 50 mg/mL | Lysozyme: 25 mg/mL |
Short term pH stability | 1-14 | 2-12 | 3-14 |
Sartobind® Q
Sartobind® Q is highly flexible and used in a wide variety of processes such as the removal of HCP, DNA, viruses, and endotoxins. It is also applied for virus and nucleic acid (DNA/RNA) purification.
As with all Sartobind® membranes, the large pore structure is particularly suited to large biomolecules.
Sartobind® Q Mini
The new Sartobind® Q Mini provides 10|20 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
Sartobind STIC® PA
Sartobind STIC® PA can operate at high conductivity and can remove DNA at up to 1.5 M NaCl. DNA removal is possible even in the presence of phosphate buffers, which offers some unique processing opportunities.
Sartobind STIC® PA Mini
The new Sartobind STIC® PA Mini provides 10 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
Sartobind® S
Sartobind® S membrane adsorber is approved for use in drug manufacturing to remove host cell proteins at high flow rates. As a cation exchanger, Sartobind® S displays aggregate removal capabilities and typically achieves at least ten-fold higher binding capacity for viruses and virus-like-particles (VLP).
Sartobind® S Mini
The new Sartobind® S Mini provides 10|20 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
Sartobind® Membranes in HIC
Sartobind® Phenyl – Easy Aggregate Removal
Sartobind® Phenyl is a hydrophobic interaction membrane with low ligand substitution. This allows for mild elution conditions for the purification of all biomolecules.
Sartobind® Phenyl membranes can be considered as a replacement to columns for polishing (flow-through) operations and a number of bind-and-elute applications, as they work at much higher flow rates, reduced complexity and without size exclusion effects when purifying large biomolecules.
The typical dynamic binding capacity (DBC) at 10 % breakthrough is:
• 9-13 mg/mL mAb
• 10 mg/mL BSA
• 23 mg/mL Lysozyme
Sartobind® Phenyl Mini
The new Sartobind® Phenyl Mini provides 20 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
