Octet® BLI Label-Free Detection Systems for Biomolecular Interactions Analysis

Unprecedented Time and Cost Savings For Kinetics, Affinity, and Concentration Assays

Based on Bio-Layer Interferometry (BLI), Octet® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation. This fluidics-free instrument platform offers several advantages over other label-free techniques like Surface Plasmon Resonance (SPR).

The Octet® BLI platform also provides robust monitoring of biomolecular interactions to overcome limitations in throughput, performance and cost associated with traditional technologies such as enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography (HPLC). These fluidics-free, low-maintenance, sensitive systems increase lab productivity, reduce costs and shorten experimental timelines.  

  • Higher Throughput - Protein quantitation in as little as two minutes, and parallel processing of up to 96 samples 
  • Fast Time to Results – Automated analysis for faster time-to-market and faster publications
  • Real-time Analysis – Binding interactions are continuously monitored as they happen. Don’t miss out on subtle kinetic behavior patterns.
  • Crude Sample Compatibility - Analytes can be measured in crude, unpurified mixtures such as cell lysates or hybridoma supernatants.
  • Ease of Use – Simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.
  • Low Total Cost of Ownership – Low-maintenance system has no fluidics modules to clog, so no downtime while waiting for replacements.

Learn More About Biolayer Interferometry (BLI) Technology

Find out how you can leverage the power of Octet®️

Boost your research and commercialization goals by combining BLI and SPR at an attractive promotional price. 

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Octet® R Series

Accelerate Your Research with an Upgradeable, Label-Free Protein Analysis Platform

The Octet® BLI is an easy-to use, real-time analytical tool that rapidly screens critical quality attributes.

2-Channel System: Octet® R2

Grows With Your Biomolecule Characterization Needs

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Octet R4 label free detection system

4-Channel System: Octet® R4

The Perfect Balance Between Cost and Throughput with Given Leeway

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Octet R8 label free detection system instrument

8-Channel System: Octet® R8

Unmatched Flexibility and Versatility in Protein Analysis

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Octet® RH16 and RH96

Unprecedented Throughput for Industry Applications

Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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Octet RH16 ultra high throughput label free detection system

16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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Octet®️ N1 – Label-Free Assays in a Drop

Octet N1 label free detection system

Octet® N1

Label-free assays in a drop

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Octet® BLI Systems in Action

See How the Octet® Bio-Layer Interferometry Systems Are Evolving

The Sartorius Octet® BLI platform has become an invaluable technique analyzing therapeutic biomolecules at various stages of development. 

With unparalleled ease-of-use, the instruments transform workflows beyond the limitations of label-free methods, providing comprehensive characterization capabilities across a broad range of applications critical for efficient biotherapeutics development and production. 

Explore the Fluidic-Free Industry Standard for Label-Free Analysis

Whether your lab is just getting started or requires higher throughput for screenings or analyzing large numbers of samples, the Octet® R series has a range of price points and throughput capabilities to help you simplify progress. 

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Discover Octet® Solutions for Your Application

With a wide range of ligand capture surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles, the Octet® BLI platform has become a must-have laboratory tool for biomolecular interactions studies. 

Benefit for Label-Free Detection Requirements

Lab technician with Octet RH96 ultra high throughput label free detection system
Experimental Simplicity to De-Risk Your Journey and Simplify Progress

A microfluidic-free design allows for unprecedented time and cost savings for binding kinetics, affinity and analyte concentration measurement with high precision, accuracy and a broad dynamic range.

Analyze Tough Samples Faster 

Parallel processing of up to 96 samples - coupled with minimal sample preparation - provides unprecedented throughput. Use fluidics-free assays with 96- and/or 384-well sample plates and ready-to-use biosensors for flexibility in sample types from large biologics to small molecules, purified or in crude form.

Perfect for Multi-User Environments 

Octet® BLI systems are known for reliable, user-friendly, trouble-free operation and have quickly become the go-to systems in academic and industrial labs for their ease-of-use, robustness and application flexibility.

Transfer Methods to QC and Manufacturing with Confidence

Rapidly develop and validate Octet® BLI assays for lot release, ligand binding, quantitation and impurity assays.

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Educational Resources

Case Study

Bispecific Antibodies: From Research to Clinical Application

Learn how Octet BLI transforms our approach to drug discovery and development

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Octet R Series System brochure cover

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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app note cover
Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

Octet® Label-Free Biosensor Analysis

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Octet Kinetics Assay Method Development Guideline thumbnail
Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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app note thumbnail
Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Product Resources

Octet top 10 reasons to choose BLI

Top Ten Reasons to Choose Sartorius Octet®️ BLI Technology to Accelerate Your Research, Development, and Manufacturing

PDF | 167.1 KB
Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications
Application Guide

Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications

PDF | 282.5 KB

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