Biologics Characterization Tools
Industry Standards for Screening, Characterizing Biomolecular Interactions (Including Antibodies)
The Octet® platform is a comprehensive solution for screening and characterizing molecular interactions such as protein-protein or protein-drug, enabling an enviable variety of applications throughout biologics development from early selection to validation to manufacturing.
Our instruments utilize industry-leading, label-free technologies - Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR). Both are deemed appropriate for bioanalysis within chapters of the United States Pharmacopeia (USP).
The Octet® family of instruments has been an industry standard for label-free analysis across biologics development and manufacturing for more than 15 years.
Discover the Octet® SF3 SPR platform
Sartorius Solutions for Biologics Characterization
Why Choose Sartorius for Label-free Biomolecular Interaction Analysis?
As a trusted scientific partner, we deliver unique solutions to research challenges. Our goal is to provide best-in-class products for real-time analysis of biomolecular interactions in micro-volume sample sizes, providing information on affinity, kinetics and concentration.
Label-free systems from Sartorius have become indispensable tools in academic and biopharmaceutical research, from characterizing binding interactions and specificity to engineering proteins and antibodies. We have more than 5000 publications on the use of Octet® products within various applications.
Our comprehensive service plans include validation, system qualification and preventative maintenance that reduce operating costs and maximize lab productivity. Our experienced team of technical support, FAS and applications development scientists are always available for research consultation.
Features of the Octet® Platform
Label-free assays measure molecular binding through real-time protein interactions. It is a precise and efficient means of collecting a wide range of information related to affinity, kinetics, concentration, thermodynamics, bio-similarity, specificity, potency and selectivity of molecular binding.
Octet® is a powerful platform designed to understand structure-function relationships between molecules, and it can be applied at many different stages throughout the drug development process.
Fluidic-free Octet® BLI assays are ideal for measurements in crude and complex matrices for instance titer and concentration analysis as well as screening for Critical Quality Attributes (CQAs) early in cell line development and bioprocess development workflows.
On the other hand, working with challenging analytes such as small fragments in FBDD, low affinity molecules or high affinity binders require a higher sensitivity that only Octet® SPR technology can offer. The patented OneStepTM gradient injection generates unique curvature to accurately detect full binding kinetics (kon, koff and KD) from a single concentration.
Label-free BLI and SPR are used from screening (at the very early stages of drug discovery) to identifying and engineering potential binding candidates, supporting clinical evaluation and finally during quality control(QC) of GMP manufacturing.
Over the last two decades, these systems have been upgraded to show significant improvements in sensitivity and software capabilities, leading to higher workflow efficiencies and more unlocked applications.
